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Showing 1–2 of 2 results
Advanced filters: Author: Julien Orlans Clear advanced filters

  • Currently many of the time resolved serial femtosecond (SFX) crystallography experiments are done with light driven protein systems, whereas the reaction initiation for non-light triggered enzymes remains a major bottle neck. Here, the authors present an expanded Drop-on-Tape system, where picoliter-sized droplets of a substrate or inhibitor are turbulently mixed with nanoliter sized droplets of microcrystal slurries, and they use it for time-resolved SFX measurements of inhibitor binding to lysozyme and secondly, binding of a β-lactam antibiotic to a bacterial serine β-lactamase.

    • Agata Butryn
    • Philipp S. Simon
    • Allen M. Orville
    ResearchOpen Access
    Nature Communications
    Volume: 12, P: 1-7

  • Serial macromolecular crystallography has become a powerful method to reveal room-temperature structures of biological macromolecules and perform time-resolved studies, however, the experiments remain complex and challenging for broader applications. Here, the authors develop serial microsecond crystallography using high-brilliance, high-repetition-rate X-ray pulses at the newly constructed ID29 beamline of the ESRF-EBS 4th generation synchrotron, featuring microsecond exposure times, innovative beam characteristics, adaptable sample environment, and high-quality complete data.

    • Julien Orlans
    • Samuel L. Rose
    • Daniele de Sanctis
    ResearchOpen Access
    Communications Chemistry
    Volume: 8, P: 1-12