The authors generated a CRISPR-Cas9 edited “workhorse” platform using a Ptf1-Cre; LSL-Cas9 mouse that. This model enables the relatively facile interrogation of the functional role of mutated genes in the pancreatic ductal adenocarcinoma landscape, including diverse combinations of targeted alleles using adeno-associated virus 8 as a delivery vector. The resulting pancreata demonstrate the full compendium of precursor and invasive lesions observed in human pancreatic ductal adenocarcinoma.
- Noboru Ideno
- Hiroshi Yamaguchi
- Bidyut Ghosh
