Flavin-dependent halogenases catalyse the challenging regioselective halogenation of aromatic compounds, but display low efficiency. Now, a tryptophan-halogenase with multiple catalytic improvements is obtained by engineering the intermediate transfer tunnel connecting the enzyme´s two active sites.
- Kridsadakorn Prakinee
- Aisaraphon Phintha
- Pimchai Chaiyen
