Iturin antimicrobial lipopeptides are a family of cyclic lipopeptides (CLPs) biosynthesized by non-ribosomal peptide synthetases (NRPSs) and comprising cyclic heptapeptides and β-amino fatty acids, however, determination of the length of the fatty acid moiety is underexplored. Here, the authors demonstrate in vivo that the acyl ligase (AL) domain in NRPS determines the length of the fatty acid moiety, and show in vitro that the mutations in the substrate-binding pocket of the AL domain affect substrate specificity.
- Rina Aoki
- Eri Kumagawa
- Shingo Kobayashi
