mRNAs encoding the regulatory components (the engineered receptor and the translational regulator) and the target mRNA are co-transfected into mammalian cells. In the absence of the ligand, the translational regulator represses target mRNA translation. Upon ligand binding, the engineered receptor facilitates the degradation of the translational regulator, leading to the activation of target mRNA translation.
- Hideyuki Nakanishi
- Keiji Itaka
