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Showing 1–5 of 5 results
Advanced filters: Author: Laura van Weeren Clear advanced filters

  • Lifetime screening of fluorescent protein variants by fluorescent lifetime imaging microscopy of bacterial colonies identifies bright, high-quantum-yield fluorescent protein variants including a cyan fluorescent protein named mTurquoise that is 1.5-fold brighter than mCerulean and has a mono-exponential fluorescence decay.

    • Joachim Goedhart
    • Laura van Weeren
    • Theodorus W J Gadella Jr
    Research
    Nature Methods
    Volume: 7, P: 137-139

  • Cyan variants of green fluorescent protein (CFPs) are widely used as donors in FRET experiments. Here, a new CFP, mTurquoise2, is developed, which displays a high-fluorescence quantum yield and a long mono-exponential fluorescence lifetime.

    • Joachim Goedhart
    • David von Stetten
    • Antoine Royant
    ResearchOpen Access
    Nature Communications
    Volume: 3, P: 1-9

  • Two mScarlet variants with high brightness and fast maturation times have been evolved. These variants behave favorably as fusion tags and Förster resonance energy transfer acceptors.

    • Theodorus W. J. Gadella Jr.
    • Laura van Weeren
    • Antoine Royant
    Research
    Nature Methods
    Volume: 20, P: 541-545

  • This protocol describes a screening pipeline to select red fluorescent proteins (FPs) with optimized parameters from a mutant FP library. FPs are screened for fluorescence lifetime, cellular brightness, maturation efficiency, and photostability.

    • Daphne S. Bindels
    • Marten Postma
    • Theodorus W. J. Gadella Jr
    Protocols
    Nature Protocols
    Volume: 15, P: 450-478