Ligand binding to GAF domains regulates the activity of associated catalytic domains in various proteins, however, the specific residues involved and the mechanism of GAF domain function remain unclear. Here, the authors combine computational and experimental approaches to demonstrate that two highly coevolving residues, L267 and F295, distant from the ligand binding site, play a critical role in GAF domain function.
- Wesam S. Ahmed
- Anupriya M. Geethakumari
- Kabir H. Biswas
