Nearly 5% of membrane proteins are 'tail-anchored' to the endoplasmic reticulum by a single carboxy-terminal transmembrane domain. These tail-anchored proteins are targeted post-translationally by the ATPase Get3, but the mechanism of recognition and targeting by Get3 is not known. Here, the crystal structures of yeast Get3 in a nucleotide-free 'open' state and a nucleotide-bound 'closed' state are presented.
- Agnieszka Mateja
- Anna Szlachcic
- Robert J. Keenan
