The authors demonstrate in vitro CRISPR/CAS9-mediated editing of the canine adenovirus type 2 (CAV2) genome to successfully insert a red fluorescent protein reporter construct and to replace E3 gene sequences with a single domain antibody in the CAV2 genome. This work provides a significantly improved and efficient method for targeted editing of adenoviruses to generate altered and potentially therapeutic viral genomes in the shortest possible time.
- Abdul Mohin Sajib
- Payal Agarwal
- Bruce F. Smith
