During pre-Replication Complex, eukaryotic cells load two MCMs into a head-to-head Double Hexamer around duplex DNA (DH). Here the authors preRC assembly assay with purified proteins to reveal insights into S. cerevisiae’s first steps that lead to the DH formation.
- Marina Guerrero-Puigdevall
- Narcis Fernandez-Fuentes
- Jordi Frigola