The existence of membrane rafts of higher lipid order in living cells is subject to ongoing debate. Here, Sevcsik et al. use a micropatterning approach to show that glycosylphosphatidylinositol-anchored proteins, typical raft constituents, do not influence their membrane nanoenvironment to promote raft phase formation.
- Eva Sevcsik
- Mario Brameshuber
- Gerhard J. Schütz