Serine recombinases are proposed to catalyse DNA site-specific recombination by a unique mechanism called subunit rotation. Here, the authors use single molecule FRET to provide direct structural and kinetic evidence for subunit rotation by two distantly related recombinases, Tn3 resolvase and Sin.
- Gillian M. Cadden
- Jan-Gero Schloetel
- W. Marshall Stark