A GFP-based reporter system has been developed to measure repair of DNA interstrand cross-links (ICLs) via homologous recombination. Cells defective in the Fanconi anemia pathway showed a marked defect in repair only when the reporter contained a replication origin, revealing an important role in ICL-induced homologous recombination coupled to replication.
- Koji Nakanishi
- Francesca Cavallo
- Maria Jasin
