Using the recently developed phosphorescent probe PtP-C343, in combination with two-photon phosphorescence lifetime microscopy, Lecoq and his colleagues offer a method for mapping oxygen levels in both microvascular and extravascular compartments with high spatial and temporal resolution. They used this set-up to make micron-scale simultaneous measurements of partial pressure of oxygen (PO2) and blood flow in the rat olfactory bulb vasculature and neuropil.
- Jérôme Lecoq
- Alexandre Parpaleix
- Serge Charpak