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Advanced filters: Author: Maxwell Zimmerley Clear advanced filters
  • This method achieves simultaneous and spatially colocalized excitation of three fluorophores with distinct spectra, doing so via two-photon microscopy using a femtosecond laser and an optical parametric oscillator and by temporally overlapping the beams. Imaging of 'Brainbow'-labeled mouse and chicken nervous tissue and of developing fly embryos is shown.

    • Pierre Mahou
    • Maxwell Zimmerley
    • Emmanuel Beaurepaire
    Research
    Nature Methods
    Volume: 9, P: 815-818