Nature Search

MacroD1 sustains mitochondrial integrity and oxidative metabolism

Here, the authors show that MacroD1 is important for mitochondrial integrity and function. Lack of MacroD1 resulted in impaired cellular respiration which was particularly detrimental for cells and organs with high energetic requirements, such as skeletal muscle.

Ann-Katrin Hopp
Lorenza P. Ferretti
Michael O. Hottiger
ResearchOpen Access15 Aug 2025
Nature Communications

Volume: 16, P: 1-19
The regulatory landscape of the human HPF1- and ARH3-dependent ADP-ribosylome

ADP-ribosylation is regulated by HPF1 and ARH3, but the cellular target spectrum of these enzymes is not fully understood. Here, the authors use quantitative proteomics to define the HPF1- and ARH3-dependent ADP-ribosylome, providing evidence that mono-ADP-ribosylation of serine predominates in cells.

Ivo A. Hendriks
Sara C. Buch-Larsen
Michael L. Nielsen
ResearchOpen Access08 Oct 2021
Nature Communications

Volume: 12, P: 1-16
Engineering Af1521 improves ADP-ribose binding and identification of ADP-ribosylated proteins

ADP-ribose binding macro domains facilitate the enrichment and detection of cellular ADP-ribosylation. Here, the authors generate an engineered macro domain with increased ADP-ribose affinity, improving the identification of ADP-ribosylated proteins by proteomics, western blot and immunofluorescence.

Kathrin Nowak
Florian Rosenthal
Michael O. Hottiger
ResearchOpen Access15 Oct 2020
Nature Communications

Volume: 11, P: 1-14
Proteome-wide identification of the endogenous ADP-ribosylome of mammalian cells and tissue

ADP-ribosylation is a reversible post-translational protein modification involved in many cellular processes. Here the authors describe a sensitive approach for the analysis of ADP-ribosylation sites under physiologic conditions and identify lysine residues as in vivotargets of ADP-ribosylation.

Rita Martello
Mario Leutert
Michael L. Nielsen
ResearchOpen Access30 Sept 2016
Nature Communications

Volume: 7, P: 1-13
Proteomic analyses identify ARH3 as a serine mono-ADP-ribosylhydrolase

Protein ADP-ribosylation has emerged as a key post translational modification that regulates several stress responses. Here the authors characterize ARH3 as a major serine-specific mono–ADP-ribosylhydrolase and use a proteomics approach to identify the cellular targets of ARH3.

Jeannette Abplanalp
Mario Leutert
Michael O. Hottiger
ResearchOpen Access12 Dec 2017
Nature Communications

Volume: 8, P: 1-11
Macrodomain-containing proteins are new mono-ADP-ribosylhydrolases

ADP-ribosylation is an important post-translational protein modification, yet enzymes capable of removing the protein-proximal ADP-ribose were unknown. Human macrodomain proteins MacroD1, D2 and C6orf130 are now shown to catalyze mono-ADP-ribose removal. MacroD2 is also shown to reverse the inhibition of GSK3A caused by ARTD10-catalyzed mono-ADP-ribosylation.

Florian Rosenthal
Karla L H Feijs
Michael O Hottiger
Research10 Mar 2013
Nature Structural & Molecular Biology

Volume: 20, P: 502-507
Covalent PARylation of DNA base excision repair proteins regulates DNA demethylation

The PARylation activity of PARP recruits DNA repair proteins to damaged DNA, most likely via non-covalent protein-PAR interactions. Here, the authors show that PARP1 covalently PARylates base excision repair proteins to modulate their DNA transactions and thus promote active BER DNA demethylation.

Simon D. Schwarz
Jianming Xu
Roland Steinacher
ResearchOpen Access02 Jan 2024
Nature Communications

Volume: 15, P: 1-13
A macrodomain-containing histone rearranges chromatin upon sensing PARP1 activation

Poly-ADP-ribosylation is a post-translational modification catalyzed by enzymes such as PARP1, which responds to metabolic and genotoxic stress. Now macrodomain-containing proteins are shown to rapidly move to PARP1 activation sites, and recruitment of the macrodomain-containing histone macroH2A1.1 results in local chromatin changes.

Gyula Timinszky
Susanne Till
Andreas G Ladurner
Research13 Aug 2009
Nature Structural & Molecular Biology

Volume: 16, P: 923-929
Transcription coactivator p300 binds PCNA and may have a role in DNA repair synthesis

Sameez Hasan
Paul O. Hassa
Michael O. Hottiger
Research15 Mar 2001
Nature

Volume: 410, P: 387-391
p53 inhibition by the LANA protein of KSHV protects against cell death

Jacques Friborg Jr
Wing-pui Kong
Gary J. Nabel
Research23 Dec 1999
Nature

Volume: 402, P: 889-894
Several posttranslational modifications act in concert to regulate gephyrin scaffolding and GABAergic transmission

Gephyrin is a cytoplasmic scaffolding protein that selectively forms postsynaptic scaffolds at GABAergic and glycinergic synapses. Here the authors characterize regulatory mechanisms determining gephyrin scaffolding and GABAA receptor synaptic transmission that involve acetylation, SUMOylation and phosphorylation.

Himanish Ghosh
Luca Auguadri
Shiva K. Tyagarajan
ResearchOpen Access07 Nov 2016
Nature Communications

Volume: 7, P: 1-16
HDAC-mediated deacetylation of NF-κB is critical for Schwann cell myelination

Using genetic approaches, this study shows that HDAC1 and HDAC2 acetylation of NF-κB is required for proper Schwann cell myelination in the peripheral nervous system and for Schwann cell differentiation.

Ying Chen
Haibo Wang
Q Richard Lu
Research20 Mar 2011
Nature Neuroscience

Volume: 14, P: 437-441
ADP-ribose-specific chromatin-affinity purification for investigating genome-wide or locus-specific chromatin ADP-ribosylation

This protocol describes how to investigate genome-wide or locus-specific chromatin ADP-ribosylation using ADPr-ChAP.

Lavinia Bisceglie
Giody Bartolomei
Michael O Hottiger
Protocols24 Aug 2017
Nature Protocols

Volume: 12, P: 1951-1961
Cytoplasmic ADP-ribosylation levels correlate with markers of patient outcome in distinct human cancers

Fabio Aimi
Holger Moch
Michael O. Hottiger
ResearchOpen Access19 Mar 2021
Modern Pathology

Volume: 34, P: 1468-1477
PARP-1 binds E2F-1 independently of its DNA binding and catalytic domains, and acts as a novel coactivator of E2F-1-mediated transcription during re-entry of quiescent cells into S phase

Cynthia M Simbulan-Rosenthal
Dean S Rosenthal
Mark E Smulson
Research20 Nov 2003
Oncogene

Volume: 22, P: 8460-8471
ARTD1 regulates osteoclastogenesis and bone homeostasis by dampening NF-κB-dependent transcription of IL-1β

Agnieszka Robaszkiewicz
Chao Qu
Michael O. Hottiger
ResearchOpen Access17 Feb 2016
Scientific Reports

Volume: 6, P: 1-14
A continuous sirtuin activity assay without any coupling to enzymatic or chemical reactions

Sabine Schuster
Claudia Roessler
Mike Schutkowski
ResearchOpen Access04 Mar 2016
Scientific Reports

Volume: 6, P: 1-12
Interplay between ADP-ribosyltransferases and essential cell signaling pathways controls cellular responses

Flurina Boehi
Patrick Manetsch
Michael O. Hottiger
ReviewsOpen Access02 Nov 2021
Cell Discovery

Volume: 7, P: 1-22
Epigenetic regulation of nitric oxide synthase 2, inducible (Nos2) by NLRC4 inflammasomes involves PARP1 cleavage

Carina de Lima Buzzo
Tiago Medina
Karina R. Bortoluci
ResearchOpen Access02 Feb 2017
Scientific Reports

Volume: 7, P: 1-12
Ecto-ADP-ribosyltransferase ARTC2.1 functionally modulates FcγR1 and FcγR2B on murine microglia

Björn Rissiek
Stephan Menzel
Tim Magnus
ResearchOpen Access28 Nov 2017
Scientific Reports

Volume: 7, P: 1-13

Search

MacroD1 sustains mitochondrial integrity and oxidative metabolism

The regulatory landscape of the human HPF1- and ARH3-dependent ADP-ribosylome

Engineering Af1521 improves ADP-ribose binding and identification of ADP-ribosylated proteins

Proteome-wide identification of the endogenous ADP-ribosylome of mammalian cells and tissue

Proteomic analyses identify ARH3 as a serine mono-ADP-ribosylhydrolase

Macrodomain-containing proteins are new mono-ADP-ribosylhydrolases

Covalent PARylation of DNA base excision repair proteins regulates DNA demethylation

A macrodomain-containing histone rearranges chromatin upon sensing PARP1 activation

Transcription coactivator p300 binds PCNA and may have a role in DNA repair synthesis

p53 inhibition by the LANA protein of KSHV protects against cell death

Several posttranslational modifications act in concert to regulate gephyrin scaffolding and GABAergic transmission

HDAC-mediated deacetylation of NF-κB is critical for Schwann cell myelination

ADP-ribose-specific chromatin-affinity purification for investigating genome-wide or locus-specific chromatin ADP-ribosylation

Cytoplasmic ADP-ribosylation levels correlate with markers of patient outcome in distinct human cancers

PARP-1 binds E2F-1 independently of its DNA binding and catalytic domains, and acts as a novel coactivator of E2F-1-mediated transcription during re-entry of quiescent cells into S phase

ARTD1 regulates osteoclastogenesis and bone homeostasis by dampening NF-κB-dependent transcription of IL-1β

A continuous sirtuin activity assay without any coupling to enzymatic or chemical reactions

Interplay between ADP-ribosyltransferases and essential cell signaling pathways controls cellular responses

Epigenetic regulation of nitric oxide synthase 2, inducible (Nos2) by NLRC4 inflammasomes involves PARP1 cleavage

Ecto-ADP-ribosyltransferase ARTC2.1 functionally modulates FcγR1 and FcγR2B on murine microglia

Search

Quick links

Search

Filter By:

Search

Quick links