DNA modifications on pyrimidine nucleobases play diverse roles in biology such as protection of bacteriophage DNA from enzymatic cleavage, however, their role in the regulation of transcription is underexplored. Here, the authors design and synthesize a series of 5-substituted pyrimidine 2ʹ-deoxyribonucleoside triphosphates and employ them for the synthesis of diverse DNA templates, showing their regulation on in vitro transcription with T7 RNA polymerase.
- Filip Gracias
- Radek Pohl
- Michal Hocek
