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Showing 1–6 of 6 results

Advanced filters: Author: Nagarjuna Nagaraj Clear advanced filters

Universal sample preparation method for proteome analysis

A method, filter-aided sample preparation (FASP) combines the advantages of in-gel and in-solution digestion for mass spectrometry–based proteomics, allowing deeper proteomic coverage in a shorter analysis time, using small sample amounts.

Jacek R Wiśniewski
Alexandre Zougman
Matthias Mann
Research19 Apr 2009
Nature Methods

Volume: 6, P: 359-362
Large-scale phosphosite quantification in tissues by a spike-in SILAC method

Quantitative, large-scale in vivo phosphoproteomics analyses are made possible with a form of spike-in stable-isotope labeling with amino acids in cell culture (SILAC), in which SILAC-labeled cell lines act as an internal standard for mass spectrometry–based tissue phosphoproteome analysis.

Mara Monetti
Nagarjuna Nagaraj
Matthias Mann
Research10 Jul 2011
Nature Methods

Volume: 8, P: 655-658
MaxDIA enables library-based and library-free data-independent acquisition proteomics

The software platform MaxDIA streamlines analysis of data-independent acquisition proteomics.

Pavel Sinitcyn
Hamid Hamzeiy
Jürgen Cox
ResearchOpen Access08 Jul 2021
Nature Biotechnology

Volume: 39, P: 1563-1573
Plausible diagnostic value of urinary isomeric dimethylarginine ratio for diabetic nephropathy

Dharmeshkumar Parmar
Nivedita Bhattacharya
Venkateswarlu Panchagnula
ResearchOpen Access19 Feb 2020
Scientific Reports

Volume: 10, P: 1-7
Minimal, encapsulated proteomic-sample processing applied to copy-number estimation in eukaryotic cells

A streamlined, robust sample-preparation method for mass spectrometry–based proteome analysis is reported. All sample preparation steps are carried out in a single enclosed reactor, reducing the potential for contamination and losses, and enabling comprehensive proteome coverage.

Nils A Kulak
Garwin Pichler
Matthias Mann
Research02 Feb 2014
Nature Methods

Volume: 11, P: 319-324
A practical guide to the MaxQuant computational platform for SILAC-based quantitative proteomics

Jürgen Cox
Ivan Matic
Matthias Mann
Protocols16 Apr 2009
Nature Protocols

Volume: 4, P: 698-705