Kinesin is a dimeric motor protein which is known to move along microtubule filaments by using its twin motor domains (heads) to carry out an asymmetric, 'hand-over-hand' walk. However, the extent of individual head interactions with the microtubule has remained controversial. Here, a single-molecule assay is developed that can directly report head binding and unbinding during kinesin movement.
- Nicholas R. Guydosh
- Steven M. Block
