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Showing 1–4 of 4 results
Advanced filters: Author: Nicolas Desprat Clear advanced filters

  • Two methods for fluorescence-based actinometry using organic dyes and photoconvertible fluorescent proteins enable rapid and precise measurement of light intensity at the sample in fluorescence microscopes.

    • Aliénor Lahlou
    • Hessam Sepasi Tehrani
    • Ludovic Jullien
    ResearchOpen Access
    Nature Methods
    Volume: 20, P: 1930-1938

  • It is unclear how cell adhesion and elongation coordinate during formation of bacterial microcolonies. Here, Duvernoy et al. monitor microcolony formation in rod-shaped bacteria, and show that patterns of surface colonization derive from the spatial distribution of adhesive factors on the cell envelope.

    • Marie-Cécilia Duvernoy
    • Thierry Mora
    • Nicolas Desprat
    ResearchOpen Access
    Nature Communications
    Volume: 9, P: 1-10

  • Label discrimination is challenging in fluorescence microscopy due to broad spectra and narrow lifetime distribution. Here, the authors introduce extra kinetic dimensions by illuminating reversibly photoswitchable fluorophores with different intensities, and discriminate 20 spectrally similar fluorophores.

    • Raja Chouket
    • Agnès Pellissier-Tanon
    • Ludovic Jullien
    ResearchOpen Access
    Nature Communications
    Volume: 13, P: 1-8