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Showing 1–5 of 5 results
Advanced filters: Author: Noah Jakimo Clear advanced filters

  • CRISPR enzymes require a defined protospacer adjacent motif (PAM) which can be limiting for editing applications. Here the authors recombine the PAM-interacting domain of SpRY with the N-terminus of Sc + + to generate a chimeric enzyme with highly flexible PAM preference: SpRYc.

    • Lin Zhao
    • Sabrina R. T. Koseki
    • Pranam Chatterjee
    ResearchOpen Access
    Nature Communications
    Volume: 14, P: 1-8

  • Cas12i is a genome editing platform with compact size that fits in AAV vector with short 43-mer gRNA, absence of tracrRNA, ability to process pre-crRNA, and high specificity. Here the authors present an unbiased mutational scanning approach to engineer Cas12i, which shows low activity in mammalian cells, and identify single substitutions that significantly improve indel activity.

    • Colin McGaw
    • Anthony J. Garrity
    • Shaorong Chong
    ResearchOpen Access
    Nature Communications
    Volume: 13, P: 1-11

  • Protospacer adjacent motif (PAM) requirements limit the target range of CRISPR endonucleases. Here, the authors graft the 5\(^{\prime}\)-NAAN-3\(^{\prime}\) PAM-interacting domain of SmacCas9 onto SpyCas9 to create adenine dinucleotide targeting chimeras.

    • Pranam Chatterjee
    • Jooyoung Lee
    • Noah Jakimo
    ResearchOpen Access
    Nature Communications
    Volume: 11, P: 1-6