Random and targeted mutagenesis of the far-red fluorescent protein Katushka followed by screening for low toxicity and red-shifted emission resulted in two near-infrared fluorescent proteins, eqFP650 and eqFP670, that display desirable properties for in vivo imaging compared to existing near-infrared fluorescent proteins.
- Dmitry Shcherbo
- Irina I Shemiakina
- Dmitriy M Chudakov