Regiospecific cyclizations of reactive poly-β-keto intermediates are known to lead to the structural variability of aromatic products of fungal nonreducing, multidomain iterative polyketide synthases (NR-PKS group of IPKSs), but questions about the process remain. The crystal structure and mutational studies of a dissected product template monodomain from PksA, the NR-PKS that initiates the biosynethesis of the hepatocarcinogen aflatoxin B1, are now presented.
- Jason M. Crawford
- Tyler P. Korman
- Craig A. Townsend