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Correlative single-molecule and structured illumination microscopy of fast dynamics at the plasma membrane

Traditional TIRF illumination is hampered by lack of precise quantification of single-molecule intensities. Here the authors combine flat-field illumination by using a standard πShaper with multi-angular TIR illumination by incorporating a spatial light modulator compatible with fast super-resolution structured illumination microscopy.

Hauke Winkelmann
Christian P. Richter
Rainer Kurre
ResearchOpen Access10 Jul 2024
Nature Communications

Volume: 15, P: 1-19
Multi-functional DNA nanostructures that puncture and remodel lipid membranes into hybrid materials

DNA nanopores can span lipid bilayers but how they interact with lipids is not known. Here the authors establish at single-molecule level the insertion mechanism and show that DNA nanopores can locally cluster and remodel membranes, and stabilize autonomously formed lipid nanotubes.

Oliver Birkholz
Jonathan R. Burns
Jacob Piehler
ResearchOpen Access18 Apr 2018
Nature Communications

Volume: 9, P: 1-12
Coordinate regulation of residual bone marrow function by paracrine trafficking of AML exosomes

J Huan
N I Hornick
P Kurre
Research25 Jun 2015
Leukemia

Volume: 29, P: 2285-2295
Autologous stem cell transplantation for high-risk Ewing's sarcoma and other pediatric solid tumors

C J Fraser
B J Weigel
M R Verneris
Research07 Nov 2005
Bone Marrow Transplantation

Volume: 37, P: 175-181
Envelope fusion protein binding studies in an inducible model of retrovirus receptor expression and in CD34⁺ cells emphasize limited transduction at low receptor levels

P Kurre
J Morris
H-P Kiem
Research25 Apr 2001
Gene Therapy

Volume: 8, P: 593-599
Rapid 1-hour transduction of whole bone marrow leads to long-term repopulation of murine recipients with lentivirus-modified hematopoietic stem cells

P Kurre
P Anandakumar
H-P Kiem
Research06 Oct 2005
Gene Therapy

Volume: 13, P: 369-373
Treatment of Fanconi anemia patients using fludarabine and low-dose TBI, followed by unrelated donor hematopoietic cell transplantation

M S Thakar
P Kurre
H-P Kiem
Research28 Jun 2010
Bone Marrow Transplantation

Volume: 46, P: 539-544
Single molecule super-resolution imaging of proteins in living Salmonella enterica using self-labelling enzymes

Britta Barlag
Oliver Beutel
Michael Hensel
ResearchOpen Access18 Aug 2016
Scientific Reports

Volume: 6, P: 1-14
Lentivirus-mediated gene transfer into hematopoietic repopulating cells in baboons

P A Horn
J C Morris
H-P Kiem
Research14 Oct 2002
Gene Therapy

Volume: 9, P: 1464-1471

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Correlative single-molecule and structured illumination microscopy of fast dynamics at the plasma membrane

Multi-functional DNA nanostructures that puncture and remodel lipid membranes into hybrid materials

Coordinate regulation of residual bone marrow function by paracrine trafficking of AML exosomes

Autologous stem cell transplantation for high-risk Ewing's sarcoma and other pediatric solid tumors

Envelope fusion protein binding studies in an inducible model of retrovirus receptor expression and in CD34⁺ cells emphasize limited transduction at low receptor levels

Rapid 1-hour transduction of whole bone marrow leads to long-term repopulation of murine recipients with lentivirus-modified hematopoietic stem cells

Treatment of Fanconi anemia patients using fludarabine and low-dose TBI, followed by unrelated donor hematopoietic cell transplantation

Single molecule super-resolution imaging of proteins in living Salmonella enterica using self-labelling enzymes

Lentivirus-mediated gene transfer into hematopoietic repopulating cells in baboons

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