2-Hydroxyacyl-CoA lyase/synthase (HACL/S) is known to catalyze the condensation of formyl-CoA with aldehydes or ketones, however, the mechanism of one-carbon elongation biochemistry is not well understood. Here, the authors report the structures of enzymes in complex with co-factors, substrates, and products, revealing key intermediates and the C-terminal active site region, and distinguishing them from related sub-families.
- Youngchang Kim
- Seung Hwan Lee
- Andrzej Joachimiak
