The development of fully defined culture conditions for human embryonic stem cells (hESCs) should enhance experimental reproducibility, reduce unwanted contaminants and facilitate scale-up production. Rodin et al. show that a substrate made of human recombinant laminin-511 supports long-term culture of hESCs and human induced pluripotent stem cells.
- Sergey Rodin
- Anna Domogatskaya
- Karl Tryggvason