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Showing 1–6 of 6 results
Advanced filters: Author: Rachel Finck Clear advanced filters

  • Mass cytometry can measure up to 34 markers on cells, but samples cannot be multiplexed. Bodenmiller et al. use metal ion tags to facilitate multiplexed mass cytometry analysis of human blood cell samples treated with 27 kinase inhibitors and 12 stimuli across a range of dosages and time points.

    • Bernd Bodenmiller
    • Eli R Zunder
    • Garry P Nolan
    Research
    Nature Biotechnology
    Volume: 30, P: 858-867

  • The work of Michael Angelo and colleagues uses multiplexed ion beam imaging (MIBI) to localize and visualize protein expression in a manner analogous to immunohistochemistry (IHC) while circumventing some of the limitations of conventional IHC with clinical samples. MIBI uses secondary ion mass spectrometry to image antibodies tagged with isotopically pure elemental metal reporters, expanding the number of targets that can be analyzed simultaneously to about 100. The approach, used here to image breast tumor tissue sections, offers over a five-log dynamic range and compatibility with standard formalin-fixed, paraffin-embedded tissue sections.

    • Michael Angelo
    • Sean C Bendall
    • Garry P Nolan
    Research
    Nature Medicine
    Volume: 20, P: 436-442

  • The ability to characterize the cellular composition and spatial organization of the tumor microenvironment has been limited by the techniques available to image the necessary number of biomarkers for broad phenotyping at a subcellular resolution. This study demonstrates the capabilities of Multiplexed Ion Beam Imaging (MIBI) for cell phenotype identification and their spatial relationships across multiple tumor types.

    • Jason Ptacek
    • Darren Locke
    • Jessica Finn
    Research
    Laboratory Investigation
    Volume: 100, P: 1111-1123