Nature Search

The Problem of the Two Types of Diamond

G. B. B. M. SUTHERLAND
D. E. BLACKWELL
W. G. SIMERAL
Research13 Nov 1954
Nature

Volume: 174, P: 901-904
Nucleotide sequence of γδ resolvase gene and demonstration that its gene product acts as a repressor of transcription

Randall R. Reed
Grant I. Shibuya
Joan A. Steitz
Research25 Nov 1982
Nature

Volume: 300, P: 381-383
Molecular programs guiding arealization of descending cortical pathways

Using cross-areal mapping of axonal projections in the mouse neocortex, we identify the subtype-specific developmental dynamics of extratelencephalic neurons and show the functional transcriptional programs driving extratelencephalic neuron diversity.

Philipp Abe
Adrien Lavalley
Denis Jabaudon
Research11 Sept 2024
Nature

Volume: 634, P: 644-651
In vivo interaction screening reveals liver-derived constraints to metastasis

Interactions between plexin B2 on hepatocytes and sempahorins on disseminated tumour cells regulate metastatic seeding in the liver.

Costanza Borrelli
Morgan Roberts
Andreas E. Moor
ResearchOpen Access24 Jul 2024
Nature

Volume: 632, P: 411-418
Transcriptional recording by CRISPR spacer acquisition from RNA

An RNA-adapting CRISPR–Cas system is coupled with amplification and sequencing steps to record, retrieve and analyse changes in the transcriptome of a bacterial cell over time.

Florian Schmidt
Mariia Y. Cherepkova
Randall J. Platt
Research03 Oct 2018
Nature

Volume: 562, P: 380-385
Fast, multiplexable and efficient somatic gene deletions in adult mouse skeletal muscle fibers using AAV-CRISPR/Cas9

Methods for somatic gene perturbation would offer advantages for screening multiple muscle gene candidates. Here the authors couple Cre-mediated skeletal muscle fiber-specific Cas9 expression with myotropic adeno-associated virus-mediated sgRNA delivery and report a system for effective somatic gene deletions in mice.

Marco Thürkauf
Shuo Lin
Markus A. Rüegg
ResearchOpen Access30 Sept 2023
Nature Communications

Volume: 14, P: 1-16
Multimodal scanning of genetic variants with base and prime editing

Thousands of rare oncogene variants are evaluated using multimodal gene editing screens.

Olivier Belli
Kyriaki Karava
Randall J. Platt
ResearchOpen Access12 Nov 2024
Nature Biotechnology

Volume: 43, P: 1458-1470
Transcriptional linkage analysis with in vivo AAV-Perturb-seq

An in vivo single-cell CRISPR screening method identifies transcriptional phenotypes of 22q11.2 deletion syndrome associated with a broad dysregulation of a class of disease susceptibility genes that are important for RNA processing and synaptic function.

Antonio J. Santinha
Esther Klingler
Randall J. Platt
ResearchOpen Access20 Sept 2023
Nature

Volume: 622, P: 367-375
Multiplexed genome engineering by Cas12a and CRISPR arrays encoded on single transcripts

A single transcript encoding Cas12a and an array of CRISPR RNAs enables multiplexed genome engineering, from multiple knockouts to transcriptional activation or repression to orthogonal transcriptional control and editing in the same sample.

Carlo C. Campa
Niels R. Weisbach
Randall J. Platt
Research12 Aug 2019
Nature Methods

Volume: 16, P: 887-893
Voices of biotech research

Nature Biotechnology asks a selection of faculty about the most exciting frontier in their field and the most needed technologies for advancing knowledge and applications.

Nasim Annabi
Matthew Baker
Jianbin Wang
Special Features10 Mar 2021
Nature Biotechnology

Volume: 39, P: 281-286
Engineered allogeneic T cells decoupling T-cell-receptor and CD3 signalling enhance the antitumour activity of bispecific antibodies

The antitumour activity of bispecific antibodies in immunocompromised hosts can be potentiated via allogeneic T cells engineered with a T-cell receptor that decouples antigen-mediated T-cell activation from T-cell cytotoxicity.

Edo Kapetanovic
Cédric R. Weber
Sai T. Reddy
ResearchOpen Access25 Sept 2024
Nature Biomedical Engineering

Volume: 8, P: 1665-1681
Author Correction: Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening

Julia Joung
Silvana Konermann
Feng Zhang
Amendments and Corrections22 Oct 2018
Nature Protocols

Volume: 14, P: 2259
Methods and applications of in vivo CRISPR screening

In vivo CRISPR screens generate high-throughput, unbiased genotype–phenotypes maps for complex biological processes that cannot be studied in vitro. This Review outlines key criteria for understanding, designing and implementing such screens and discusses their potential impact on basic and translational research.

Antonio J. Santinha
Alessio Strano
Randall J. Platt
Reviews29 Jul 2025
Nature Reviews Genetics

Volume: 26, P: 702-718
CRISPR tool modifies genes precisely by copying RNA into the genome

The ultimate goal of genome editing is to be able to make any specific change to the blueprint of life. A ‘search-and-replace’ method for genome editing takes us a giant leap closer to this ambitious goal.

Randall J. Platt
News & Views11 Nov 2019
Nature

Volume: 576, P: 48-49
Temporal controls over inter-areal cortical projection neuron fate diversity

Combined analysis of the connectome and transcriptome in the mouse cortex indicates that dynamic differences in expression levels of largely generic sets of genes regulate differential targeting within neuronal subtypes.

Esther Klingler
Ugo Tomasello
Denis Jabaudon
Research09 Nov 2021
Nature

Volume: 599, P: 453-457
AAV-mediated direct in vivo CRISPR screen identifies functional suppressors in glioblastoma

Using an adeno-associated virus–mediated, direct in vivo CRISPR screen, the authors mapped a quantitative landscape of glioblastoma suppressors. Their study revealed gene combinations that functionally drive gliomagenesis from normal glia in native mouse brains. The authors further demonstrate that mutational background can differentially influence gene expression and chemotherapeutic resistance.

Ryan D Chow
Christopher D Guzman
Sidi Chen
Research14 Aug 2017
Nature Neuroscience

Volume: 20, P: 1329-1341
Efficient CRISPR-Cas9–mediated genome editing in Plasmodium falciparum

Single guide RNAs driven by a T7 promoter target Cas9 to two endogenous loci, leading to fast and efficient genome editing in the malaria parasite P. falciparum.

Jeffrey C Wagner
Randall J Platt
Jacquin C Niles
Research10 Aug 2014
Nature Methods

Volume: 11, P: 915-918
Optical control of mammalian endogenous transcription and epigenetic states

Here the customizable TALE DNA-binding domain was integrated with the light-sensitive cryptochrome 2 protein and its interacting partner (CIB1) from Arabidopsis thaliana, thereby creating an optogenetic two-hybrid system called light-inducible transcriptional effectors (LITEs); the LITE system establishes a novel mode of optogenetic control of endogenous transcription and epigenetic states.

Silvana Konermann
Mark D. Brigham
Feng Zhang
Research23 Aug 2013
Nature

Volume: 500, P: 472-476
Integrated compact regulators of protein activity enable control of signaling pathways and genome-editing in vivo

Nik Franko
António José da Silva Santinha
Martin Fussenegger
ResearchOpen Access23 Jan 2024
Cell Discovery

Volume: 10, P: 1-14
Recording transcriptional histories using Record-seq

Record-seq is a technology that enables transcriptional recording by CRISPR spacer acquisition from RNA. Transcriptomes are recovered using SENECA, a method that selectively amplifies expanded CRISPR arrays, followed by deep sequencing.

Tanmay Tanna
Florian Schmidt
Randall J. Platt
Protocols10 Jan 2020
Nature Protocols

Volume: 15, P: 513-539
Therapeutic genome editing: prospects and challenges

David Benjamin Turitz Cox
Randall Jeffrey Platt
Feng Zhang
Reviews05 Feb 2015
Nature Medicine

Volume: 21, P: 121-131
Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening

This protocol from Feng Zhang's lab enables genome-scale knockout and transcriptional activation screening using the CRISPR-Cas9 system, as sgRNA libraries are constructed and packaged into lentiviral vectors for delivery into cells for screening.

Julia Joung
Silvana Konermann
Feng Zhang
Protocols23 Mar 2017
Nature Protocols

Volume: 12, P: 828-863

Search

The Problem of the Two Types of Diamond

Nucleotide sequence of γδ resolvase gene and demonstration that its gene product acts as a repressor of transcription

Molecular programs guiding arealization of descending cortical pathways

In vivo interaction screening reveals liver-derived constraints to metastasis

Transcriptional recording by CRISPR spacer acquisition from RNA

Fast, multiplexable and efficient somatic gene deletions in adult mouse skeletal muscle fibers using AAV-CRISPR/Cas9

Multimodal scanning of genetic variants with base and prime editing

Transcriptional linkage analysis with in vivo AAV-Perturb-seq

Multiplexed genome engineering by Cas12a and CRISPR arrays encoded on single transcripts

Voices of biotech research

Engineered allogeneic T cells decoupling T-cell-receptor and CD3 signalling enhance the antitumour activity of bispecific antibodies

Author Correction: Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening

Methods and applications of in vivo CRISPR screening

CRISPR tool modifies genes precisely by copying RNA into the genome

Temporal controls over inter-areal cortical projection neuron fate diversity

AAV-mediated direct in vivo CRISPR screen identifies functional suppressors in glioblastoma

Efficient CRISPR-Cas9–mediated genome editing in Plasmodium falciparum

Optical control of mammalian endogenous transcription and epigenetic states

Integrated compact regulators of protein activity enable control of signaling pathways and genome-editing in vivo

Recording transcriptional histories using Record-seq

Therapeutic genome editing: prospects and challenges

Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening

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