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Showing 1–5 of 5 results
Advanced filters: Author: Rene Yu-Hong Cheng Clear advanced filters

  • Linking proteins secreted from individual cells with other cellular information is challenging. Here, authors report a high-throughput method which uses hydrogel nanovials loaded with single cells to link the secretion profile of individual cells with their surface markers and transcriptomic data.

    • Rene Yu-Hong Cheng
    • Joseph de Rutte
    • Richard G. James
    ResearchOpen Access
    Nature Communications
    Volume: 14, P: 1-15

  • Plasma B cells (PC) are a potential source for protein replacement as they could be engineered to secrete protein other than antibody. Here the authors engineer B cells to express exogenous proteins and demonstrate that these cells can persist long term in adoptive transfer experiments in mice.

    • Rene Yu-Hong Cheng
    • King L. Hung
    • Richard G. James
    ResearchOpen Access
    Nature Communications
    Volume: 13, P: 1-14

  • SEC-seq is a single-cell method linking the secretion of a target protein to the cell’s transcriptome. Nanovials isolate cells and capture their secretions, and oligonucleotide-labeled antibodies tag them, followed by an scRNA-seq readout.

    • Justin Langerman
    • Sevana Baghdasarian
    • Dino Di Carlo
    Protocols
    Nature Protocols
    Volume: 20, P: 2034-2055

  • In an inter-laboratory study, the authors compare the accuracy and performance of three optical density calibration protocols (colloidal silica, serial dilution of silica microspheres, and colony-forming unit (CFU) assay). They demonstrate that serial dilution of silica microspheres is the best of these tested protocols, allowing precise and robust calibration that is easily assessed for quality control and can also evaluate the effective linear range of an instrument.

    • Jacob Beal
    • Natalie G. Farny
    • Jiajie Zhou
    ResearchOpen Access
    Communications Biology
    Volume: 3, P: 1-29