Tryptic digestion of SUMOylated proteins generates large peptides, rendering proteomic characterisation of this post-translational modification particularly challenging unless mutant SUMO is used. Hendriks et al.present a method that allows the quantitative identification of wild-type SUMO sites.
- Ivo A. Hendriks
- Rochelle C. D’Souza
- Alfred C. O. Vertegaal