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Showing 1–20 of 20 results
Advanced filters: Author: Rodrigo Ledesma-Amaro Clear advanced filters

  • Microorganisms can be engineered to sustainably produce a variety of products including fuels, pharmaceuticals, materials, and food. This work reports the design and creation of syntrophic synthetic communities of single and multiple yeast species, which achieve improved bioproduction.

    • Young-Kyoung Park
    • Huadong Peng
    • Rodrigo Ledesma-Amaro
    ResearchOpen Access
    Nature Communications
    Volume: 15, P: 1-10

  • CRISPR gene activation and inhibition has become a powerful synthetic tool for influencing the expression of native genes for foundational studies, cellular reprograming, and metabolic engineering. Here the authors demonstrate near leak-free, inducible expression of a polycistronic array containing up to 24 gRNAs from two orthogonal CRISPR/Cas systems.

    • William M. Shaw
    • Lucie Studená
    • Rodrigo Ledesma-Amaro
    ResearchOpen Access
    Nature Communications
    Volume: 13, P: 1-10

  • Microbes frequently encounter suboptimal conditions. Here, Shabestary et al. show that phenotypic heterogeneity is an important feature of Saccharomyces species adaptation where amino acid identity serves as an environmental cue driving this adaptive process.

    • Kiyan Shabestary
    • Cinzia Klemm
    • Rodrigo Ledesma-Amaro
    ResearchOpen Access
    Nature Communications
    Volume: 15, P: 1-16

  • Rapid diagnosis of bloodstream infections is vital for timely effective treatment. Here, Mao and Wan et al developed a method using a dual stem-loop DNA signal amplifier, to achieve ultrasensitive, rapid, amplification-free detection of pathogens from human serum.

    • Huiyou Chen
    • Fengge Song
    • Chuanbin Mao
    ResearchOpen Access
    Nature Communications
    Volume: 16, P: 1-14

  • Systematically culturing combinations of auxotrophic yeast mutants leads to the identification of pairs that form obligatory cross-feeding relationships, some of which are stable over time and can divide metabolic labor for biotechnological applications.

    • Simran Kaur Aulakh
    • Lara Sellés Vidal
    • Rodrigo Ledesma-Amaro
    ResearchOpen Access
    Nature Chemical Biology
    Volume: 19, P: 951-961

  • Biomolecular condensates can serve as synthetic organelle-like compartments within prokaryotes. Here, the authors report a synthetic condensate platform, generated through the incorporation of artificial, disordered proteins, and demonstrate control of metabolic flux and protein translation in Bacillus subtilis.

    • Wenwen Yu
    • Ke Jin
    • Long Liu
    ResearchOpen Access
    Nature Communications
    Volume: 15, P: 1-13

  • Rubusoside and rebaudiosides are considered the next generation of sugar substitutes. In this article, the authors report the engineering of Saccharomyces cerevisiae, remodelling the complex metabolic networks by a modular engineering approach, obtaining rubusoside and rebaudiosides at titers of around 1.4 g/L and 100 mg/L, respectively.

    • Yameng Xu
    • Xinglong Wang
    • Long Liu
    ResearchOpen Access
    Nature Communications
    Volume: 13, P: 1-12

  • Pyruvate-responsive circuits based on an orthologous transcription factor and adaptation of an antisense transcriptional circuit were developed to sense pyruvate in Bacillus subtilis and redirect metabolism for optimized glucaric acid production.

    • Xianhao Xu
    • Xueliang Li
    • Long Liu
    Research
    Nature Chemical Biology
    Volume: 16, P: 1261-1268

  • A survey of natural metabolic pathways for C1 substrates (methane, methanol, carbon dioxide, and carbon monoxide) highlights past efforts and recent progress, and informs future opportunities to create synthetic C1-utilizing microbes.

    • Wei Jiang
    • David Hernández Villamor
    • Rodrigo Ledesma-Amaro
    Reviews
    Nature Chemical Biology
    Volume: 17, P: 845-855

  • In this Review article, the authors discuss the potential of microorganisms as a solution to the challenges faced by our food system. Engineered microorganisms can be used to produce enhanced foods and ingredients in a sustainable manner. The technical, economical, and societal limitations are also discussed together with the current and future perspectives.

    • Alicia E. Graham
    • Rodrigo Ledesma-Amaro
    ReviewsOpen Access
    Nature Communications
    Volume: 14, P: 1-10

  • Improving photosynthetic efficiency is crucial for CO>sub<2 > /sub < -based biomanufacturing and agriculture. Here, biomass-derived carbon dots in biohybrids are shown to enhance natural photosynthesis by converting solar irradiation to red light and supplying electrons to the photosynthetic electron transfer chain.

    • Wenbo Cheng
    • Xueyun Wang
    • Xiang Gao
    ResearchOpen Access
    Communications Materials
    Volume: 6, P: 1-13

  • Cell engineering by synthetic biology typically relies on synthetic gene constructs that compete with the host cell for intracellular resources. This Review discusses how such resource competition can impact mammalian cell engineering and outlines strategies for how to mitigate cellular burden using circuit-centric and host-centric approaches.

    • Roberto Di Blasi
    • Jacopo Gabrielli
    • Francesca Ceroni
    Reviews
    Nature Reviews Bioengineering
    Volume: 2, P: 721-732

  • Using CRISPR/Cas9 technology and Golden Gate Assembly, a toolkit including 147 plasmids and 137 promoters enables chromosomal integration of markerless DNA and easy assembly of new gRNAs, effectively proven in a novel Y. lipolytica production strain.

    • Tigran V. Yuzbashev
    • Evgeniya Y. Yuzbasheva
    • Rodrigo Ledesma-Amaro
    ResearchOpen Access
    Communications Biology
    Volume: 6, P: 1-11

  • Multiplexed CRISPR technologies have recently emerged as powerful approaches for genetic editing and transcriptional regulation. Here the authors review this emerging technology and discuss challenges and considerations for future studies.

    • Nicholas S. McCarty
    • Alicia E. Graham
    • Rodrigo Ledesma-Amaro
    ReviewsOpen Access
    Nature Communications
    Volume: 11, P: 1-13