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High-resolution structural and functional deep brain imaging using adaptive optics three-photon microscopy

Three-photon microscopy in combination with adaptive optics-based aberration correction and ECG-triggered gating allows high-resolution imaging of neurons and astrocytes up to a depth of 1.4 mm in the mouse brain.

Lina Streich
Juan Carlos Boffi
Robert Prevedel
ResearchOpen Access30 Sept 2021
Nature Methods

Volume: 18, P: 1253-1258

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High-resolution structural and functional deep brain imaging using adaptive optics three-photon microscopy

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