This is a Protocol for fluorescent intrabody localization microscopy imaging, which enables single-particle tracking of proteins in living cells, using unique nanobodies to capture protein conformer subpopulations. This is followed by nanoclustering analysis using spatiotemporal indexing to analyze their spatiotemporal clustering.
- Rachel S. Gormal
- Tristan P. Wallis
- Frédéric A. Meunier
