Sebastian Maerkl and colleagues use single-cell measurements to show that the output of the yeast PHO5 promoter can be precisely tuned by subtle changes in binding-site affinity for the Pho4 transcription factor. These results provide insights into how transcription-factor binding sites regulate gene expression and demonstrate the feasibility of developing quantitative models of transcriptional regulatory networks.
- Arun S Rajkumar
- Nicolas Dénervaud
- Sebastian J Maerkl
