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Author Correction: Z-REX: shepherding reactive electrophiles to specific proteins expressed tissue specifically or ubiquitously, and recording the resultant functional electrophile-induced redox responses in larval fish

Kuan-Ting Huang
Jesse R. Poganik
Yimon Aye
Amendments and Corrections20 Jun 2023
Nature Protocols

Volume: 18, P: 3155
Akt3 is a privileged first responder in isozyme-specific electrophile response

The use of the T-REX redox targeting platform to identify proteins that react with lipid-derived electrophiles in cells and zebrafish reveals that hydroxynoneal (HNE)-mediated modification of Akt3 C119 decreases Akt3 activity.

Marcus J C Long
Saba Parvez
Yimon Aye
Research23 Jan 2017
Nature Chemical Biology

Volume: 13, P: 333-338
Wdr1 and cofilin are necessary mediators of immune-cell-specific apoptosis triggered by Tecfidera

The mechanism-of-action of many electrohilic drugs remains poorly understood. Here, the authors use a redox-targeting approach to elucidate the basis for the innate immune cell toxicity of dimethyl fumarate, showing that it modifies Keap1 to trigger mitochondrial-targeted neutrophil/macrophage apoptosis.

Jesse R. Poganik
Kuan-Ting Huang
Yimon Aye
ResearchOpen Access30 Sept 2021
Nature Communications

Volume: 12, P: 1-15
T-REX on-demand redox targeting in live cells

Investigating the ramifications of site-specific protein redox modification in cells is challenging. This protocol uses HaloTagged proteins and a HaloTag-targetable photocaged 4-hydroxynonenal to elicit target-specific modifications and to trace their effects.

Saba Parvez
Marcus J C Long
Yimon Aye
Protocols27 Oct 2016
Nature Protocols

Volume: 11, P: 2328-2356
Large-scale F0 CRISPR screens in vivo using MIC-Drop

This protocol for Multiplexed Intermixed CRISPR Droplets uses microfluidics to create droplets containing Cas9, multiplexed single-guide RNAs and corresponding DNA barcodes, allowing large-scale genetic screens to be performed in F0 zebrafish.

Saba Parvez
Zachary J. Brandt
Randall T. Peterson
Protocols17 Apr 2023
Nature Protocols

Volume: 18, P: 1841-1865
Z-REX: shepherding reactive electrophiles to specific proteins expressed tissue specifically or ubiquitously, and recording the resultant functional electrophile-induced redox responses in larval fish

This Protocol Extension describes Zebrafish targeting of Reactive Electrophiles and oXidants (Z-REX), an extension of a Protocol on the targetable reactive electrophiles and oxidants, adapted for on-demand redox targeting in live zebrafish embryos. The protocol details how to generate transgenic fish and run Z-REX, as well as its downstream validation.

Kuan-Ting Huang
Jesse R. Poganik
Yimon Aye
Protocols05 Apr 2023
Nature Protocols

Volume: 18, P: 1379-1415

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Author Correction: Z-REX: shepherding reactive electrophiles to specific proteins expressed tissue specifically or ubiquitously, and recording the resultant functional electrophile-induced redox responses in larval fish

Akt3 is a privileged first responder in isozyme-specific electrophile response

Wdr1 and cofilin are necessary mediators of immune-cell-specific apoptosis triggered by Tecfidera

T-REX on-demand redox targeting in live cells

Large-scale F0 CRISPR screens in vivo using MIC-Drop

Z-REX: shepherding reactive electrophiles to specific proteins expressed tissue specifically or ubiquitously, and recording the resultant functional electrophile-induced redox responses in larval fish

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