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Showing 1–3 of 3 results

Advanced filters: Author: Sanae Ogiwara Clear advanced filters

A transposon-based chromosomal engineering method to survey a large cis-regulatory landscape in mice

Kokubu and colleagues have developed a method for mapping cis-regulatory elements in the mouse using targeted integration of the Sleeping Beauty transposon. This method also gives researchers the ability to generate targeted deletions for testing loss-of-function effects.

Chikara Kokubu
Kyoji Horie
Junji Takeda
Research26 Jul 2009
Nature Genetics

Volume: 41, P: 946-952
Creation of CRISPR-based germline-genome-engineered mice without ex vivo handling of zygotes by i-GONAD

This protocol describes procedures for generating genome-edited mouse models by injecting CRISPR reagents into oviducts of pregnant females and subsequently electroporating the reagents into zygotes in situ, thus bypassing cumbersome ex vivo handling of embryos.

Channabasavaiah B. Gurumurthy
Masahiro Sato
Masato Ohtsuka
Protocols24 Jul 2019
Nature Protocols

Volume: 14, P: 2452-2482
Behavior of DNA-lacking mitochondria in Entamoeba histolytica revealed by organelle transplant

Makoto Kazama
Sanae Ogiwara
Hiroshi Tachibana
ResearchOpen Access13 Mar 2017
Scientific Reports

Volume: 7, P: 1-10