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Showing 1–6 of 6 results
Advanced filters: Author: Sara Zaccara Clear advanced filters

  • Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that cite the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, although such publications are still vastly outnumbered by those that do not.

    • Stephen A Bustin
    • Vladimir Benes
    • Jo Vandesompele
    Comments & Opinion
    Nature Methods
    Volume: 10, P: 1063-1067

  • Pepper, an RNA aptamer that prevents degradation of degron-tagged fluorescent proteins, enables fully genetically encoded fluorescence imaging of mRNA in living cells.

    • Jiahui Wu
    • Sara Zaccara
    • Samie R. Jaffrey
    Research
    Nature Methods
    Volume: 16, P: 862-865

  • The cytosolic N6-methyladenosine (m6A)-binding proteins YTHDF1, YTHDF2 and YTHDF3 undergo liquid–liquid phase separation in vitro and in cells; this is enhanced by polymethylated mRNAs to form complexes that partition into different cellular phase-separated compartments.

    • Ryan J. Ries
    • Sara Zaccara
    • Samie R. Jaffrey
    Research
    Nature
    Volume: 571, P: 424-428

  • N 6-methyladenosine (m6A) is the most abundant mRNA internal modification. The recent mapping of m6A has provided insights into which and how mRNAs are modified, how m6A affects gene expression and how it is linked to cellular differentiation, cancer progression and other biological processes.

    • Sara Zaccara
    • Ryan J. Ries
    • Samie R. Jaffrey
    Reviews
    Nature Reviews Molecular Cell Biology
    Volume: 20, P: 608-624