Nature Search

Mapping and engineering RNA-driven architecture of the multiphase nucleolus

Spatially segregated rRNA processing dictates nucleolar morphology and drives outward progression of pre-ribosomal RNA through nucleolar phases.

Sofia A. Quinodoz
Lifei Jiang
Clifford P. Brangwynne
ResearchOpen Access02 Jul 2025
Nature

Volume: 644, P: 557-566
Stage-specific assembly events of the 6-MDa small-subunit processome initiate eukaryotic ribosome biogenesis

A proteomics approach using MS2 as an RNA tag is used to provide snapshots of nascent preribosomal particles from budding yeast, thus allowing the determination of the stage-specific order in which 70 ribosome-assembly factors associate with pre-rRNA domains.

Malik Chaker-Margot
Mirjam Hunziker
Sebastian Klinge
Research19 Oct 2015
Nature Structural & Molecular Biology

Volume: 22, P: 920-923
Modular assembly of the nucleolar pre-60S ribosomal subunit

Cryo-electron microscopy structures of multiple states along the assembly pathway of the nucleolar pre-60S ribosomal subunit suggest that folding of the pre-ribosomal RNA and sequential binding of transient assembly factors are regulated by steric hindrance among these factors.

Zahra Assur Sanghai
Linamarie Miller
Sebastian Klinge
Research05 Mar 2018
Nature

Volume: 556, P: 126-129
Principles of mitoribosomal small subunit assembly in eukaryotes

The structures of both human and yeast mitochondrial ribosomal small subunits undergoing assembly are uncovered.

Nathan J. Harper
Chloe Burnside
Sebastian Klinge
ResearchOpen Access08 Dec 2022
Nature

Volume: 614, P: 175-181
A co-transcriptional ribosome assembly checkpoint controls nascent large ribosomal subunit maturation

Here, using cryo-EM, the authors detail how the yeast co-transcriptional assembly of the large ribosomal subunit involves a quality-control checkpoint. Ribosome-assembly factors implement this checkpoint by probing the formation and ensuring the co-operative stabilization of correctly folded ribosomal RNA.

Zahra A. Sanghai
Rafal Piwowarczyk
Sebastian Klinge
ResearchOpen Access10 Apr 2023
Nature Structural & Molecular Biology

Volume: 30, P: 594-599
Evolutionary compaction and adaptation visualized by the structure of the dormant microsporidian ribosome

The cryo-electron microscopy structure of the ribosome from the microsporidium Vairimorpha necatrix reveals that, despite extreme genome compaction reducing the ribosomal RNA to a functionally conserved core, most ribosomal proteins are retained and adapt to form the minimized protein synthesis machinery in these eukaryotic parasites.

Jonas Barandun
Mirjam Hunziker
Sebastian Klinge
Research22 Jul 2019
Nature Microbiology

Volume: 4, P: 1798-1804
UtpA and UtpB chaperone nascent pre-ribosomal RNA and U3 snoRNA to initiate eukaryotic ribosome assembly

Eukaryotic ribosome biogenesis involves a large number of maturations factors which are responsible for the stepwise assembly of the ribosomal subunits. Here the authors use an array of biochemical and structural biology methods to investigate the function of the UtpA and UtpB complexes as part of the small subunit processome.

Mirjam Hunziker
Jonas Barandun
Sebastian Klinge
ResearchOpen Access29 Jun 2016
Nature Communications

Volume: 7, P: 1-10
An iron-sulfur domain of the eukaryotic primase is essential for RNA primer synthesis

Sebastian Klinge
Judy Hirst
Luca Pellegrini
Research19 Aug 2007
Nature Structural & Molecular Biology

Volume: 14, P: 875-877
The complete structure of the small-subunit processome

The 3.8-Å cryo-EM structure of the Saccharomyces cerevisiae small-subunit processome in a state that precedes pre-rRNA cleavage at site A1 provides an essentially complete near-atomic model of this assembly.

Jonas Barandun
Malik Chaker-Margot
Sebastian Klinge
Research25 Sept 2017
Nature Structural & Molecular Biology

Volume: 24, P: 944-953
Rapid clonal identification of biallelic CRISPR/Cas9 knock-ins using SNEAK PEEC

Sameer Singh
Anoosha Banerjee
Sebastian Klinge
ResearchOpen Access31 Jan 2023
Scientific Reports

Volume: 13, P: 1-7
Ribosome assembly coming into focus

Our understanding of eukaryotic ribosome assembly has been boosted by recently published cryo-electron microscopy structures of yeast ribosome assembly intermediates. These studies highlight the roles of RNA compaction, checkpoints and proofreading mechanisms of pre-ribosomal particles in the nucleolus, nucleus and cytoplasm.

Sebastian Klinge
John L. Woolford Jr
Reviews22 Nov 2018
Nature Reviews Molecular Cell Biology

Volume: 20, P: 116-131
A single administration of the antibiotic, minocycline, reduces fear processing and improves implicit learning in healthy volunteers: analysis of the serum metabolome

Shi Yu Chan
Liliana Capitão
Philip W. J. Burnet
ResearchOpen Access13 May 2020
Translational Psychiatry

Volume: 10, P: 1-11
Identification of regulatory variants associated with genetic susceptibility to meningococcal disease

Lisa Borghini
Eileen Png
Peter Schermann
ResearchOpen Access06 May 2019
Scientific Reports

Volume: 9, P: 1-15
Increased GABAergic Efficacy of Central Amygdala Projections to Neuropeptide S Neurons in the Brainstem During Fear Memory Retrieval

Kay Jüngling
Maren D Lange
Hans-Christian Pape
Research04 May 2015
Neuropsychopharmacology

Volume: 40, P: 2753-2763

Search

Filter By:

Search

Quick links