3D hybrid-micromesh assisted bioprinting (Hy-MAP) method combines 3D-printed micromesh scaffold structures and sequential hydrogel patterning for multi-cellular coculture models. This method of bioprinting enables rapid cell coculture through the allowance of various methods, including injection, dipping and draining. By using this method, vascularized tumor spheroids were formed by culturing endothelial cells, stromal cell mixtures and tumor spheroids inside separate but adjacent compartments. The novel approach described in this work will provide an alternative method for fabricating mesoscale implantable tissue engineering constructs and organ-on-a-chip applications.
- Byungjun Lee
- Suryong Kim
- Noo Li Jeon
