Metamorphic proteins can switch reversibly between two different native folds with entirely distinct functions, however, the triggers that induce the conformational switch remain largely underexplored. Here, the authors demonstrate the impact of a crowded environment on the switch of two metamorphic proteins KaiB and XCL1, and study the kinetics and thermodynamics of the switch using NMR spectroscopy.
- Ning Zhang
- Wenyan Guan
- Nana Ai
