Nature Search

Mass photometric detection and quantification of nanoscale α-synuclein phase separation

The mechanism of α-synuclein amyloid aggregation via liquid–liquid phase separation has so far remained elusive. Now, the existence of nanoscale clusters of α-synuclein in sub-saturated concentrations is observed using mass photometry. These nanoscale clusters can act as precursors to both macroscopic condensate droplets as well as amyloid fibrils.

Soumik Ray
Thomas O. Mason
Alexander K. Buell
Research19 Jun 2023
Nature Chemistry

Volume: 15, P: 1306-1316
Capillary flow experiments for thermodynamic and kinetic characterization of protein liquid-liquid phase separation

Methods to quantitatively study liquid-liquid phase separation (LLPS) of proteins are lacking. Here the authors report Capillary flow experiments (Capflex) for the quantification of key LLPS parameters; they study Ddx4, the RP3 peptide and the aberrant liquid-to-solid phase transition of α-synuclein.

Emil G. P. Stender
Soumik Ray
Alexander K. Buell
ResearchOpen Access15 Dec 2021
Nature Communications

Volume: 12, P: 1-18
α-Synuclein aggregation nucleates through liquid–liquid phase separation

The mechanism of nucleation for α-synuclein (α-Syn) aggregation and amyloid formation in Parkinson’s disease is unclear. Now, α-Syn has been shown to undergo liquid–liquid phase separation and a liquid-to-solid-like transition leading to amyloid fibril formation. This raises the possibility that liquid–liquid phase separation is a key pathogenic mechanism behind α-Syn aggregation in Parkinson’s disease.

Soumik Ray
Nitu Singh
Samir K. Maji
Research08 Jun 2020
Nature Chemistry

Volume: 12, P: 705-716
Intermolecular interactions underlie protein/peptide phase separation irrespective of sequence and structure at crowded milieu

In this work, the authors report that proteins/polypeptides with diverse sequences and structures can form liquid condensates, however with varying phase regimes and different modes of intermolecular interactions, spanning from electrostatic, hydrophobic and H-bond.

Manisha Poudyal
Komal Patel
Samir K. Maji
ResearchOpen Access04 Oct 2023
Nature Communications

Volume: 14, P: 1-21
Predictable phase-separated proteins

An approach to design artificial intrinsically disordered proteins using a short peptide as a repeating unit has been reported. This design enables the phase behaviour of the protein to be finely tuned inside cells and enabled the formation of phase-separated condensates that can modulate chemical reactions.

Soumik Ray
Samir K. Maji
News & Views17 Aug 2020
Nature Chemistry

Volume: 12, P: 787-789
Evaluation of management practices in rice–wheat cropping system using multicriteria decision-making methods in conservation agriculture

Tufleuddin Biswas
Anurup Majumder
Adelajda Matuka
ResearchOpen Access13 Apr 2024
Scientific Reports

Volume: 14, P: 1-12

Search

Filter By:

Mass photometric detection and quantification of nanoscale α-synuclein phase separation

Capillary flow experiments for thermodynamic and kinetic characterization of protein liquid-liquid phase separation

α-Synuclein aggregation nucleates through liquid–liquid phase separation

Intermolecular interactions underlie protein/peptide phase separation irrespective of sequence and structure at crowded milieu

Predictable phase-separated proteins

Evaluation of management practices in rice–wheat cropping system using multicriteria decision-making methods in conservation agriculture

Search

Quick links