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Showing 1–4 of 4 results
Advanced filters: Author: Sven Klumpe Clear advanced filters

  • Serial Lift-Out creates a series of lamellae from one lift-out volume for cryo-ET, increasing the ease and throughput of cryo-lift-out and enabling the study of molecular anatomy in multicellular systems including C. elegans larvae.

    • Oda Helene Schiøtz
    • Christoph J. O. Kaiser
    • Jürgen M. Plitzko
    ResearchOpen Access
    Nature Methods
    Volume: 21, P: 1684-1692

  • The study of insulating material by scanning electron microscopy (SEM) is limited by charging artefacts. Here, the authors report an interleaved scanning approach on frozen-hydrated biological samples that fosters charge dissipation and attenuates artefacts.

    • Abner Velazco
    • Thomas Glen
    • Maud Dumoux
    ResearchOpen Access
    Nature Communications
    Volume: 16, P: 1-13

  • The large and small subunits of the ribosome are synthesized independently within the nucleolus — a membrane-less compartment within the nucleus — before being exported into the cytoplasm. Here, the authors use in situ cryo-ET to observe ribosome maturation and reveal the native organization of the nucleolus.

    • Philipp S. Erdmann
    • Zhen Hou
    • Wolfgang Baumeister
    ResearchOpen Access
    Nature Communications
    Volume: 12, P: 1-9

  • The interplay of the proteasome and deubiquitinase Ubp6 is crucial for the degradation of ubiquitylated substrates. Here, the authors provide structural insights into the allosteric mechanism by which the activities of both Ubp6 and the proteasome are regulated.

    • Ka Ying Sharon Hung
    • Sven Klumpe
    • Daniel Finley
    ResearchOpen Access
    Nature Communications
    Volume: 13, P: 1-13