Inhibition of proteins other than the intended target by small molecules can lead to the incorrect assignment of biological functions to particular proteins and wasted drug development efforts. Potential inhibition of off-target kinases by kinase inhibitors is often investigated, but kinase inhibitors can also inhibit non-kinases. Munoz examines the growing number of examples of this issue and suggests a systematic strategy to verify which protein is responsible for the effects of a given small molecule.
