Kinase inhibitors are key in cancer therapy, but resistance limits their efficacy. Here, the authors develop SPIED-DIA, a phosphoproteomics method enhancing detection of key phosphorylation sites. They reveal a synergistic MEK-JNK signaling response in colorectal cancer cells, suggesting a potential therapeutic strategy.
- Mirjam van Bentum
- Bertram Klinger
- Matthias Selbach
