Vanadium-dependent haloperoxidases (VHPOs) are versatile enzymes that catalyze oxidation of halides followed by halogenation of electron-rich substrates, but the factors dictating substrate and halogen specificity were unknown. Here, the authors show that a single-point mutation in the cyanobacterial bromoperoxidase AmVHPO facilitates a selectivity switch to allow aryl chlorination and elucidate the structural basis for this switch.
- P. Zeides
- K. Bellmann-Sickert
- T. Gulder
