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Leveraging relaxation-optimized ¹H–¹³C_F correlations in 4-¹⁹F-phenylalanine as atomic beacons for probing structure and dynamics of large proteins

Correlating aromatic carbons attached to fluorine with meta-position hydrogens in fluorine-labelled phenylalanines can yield two-dimensional correlations with narrow linewidths in large proteins. Adapting phenylalanine-tRNA synthetase increases the incorporation rate, while expanding the genetic code enables site-specific incorporation of fluorinated phenylalanine. The resulting HC_F-transverse relaxation-optimized spectroscopy can illuminate protein dynamics and drive multiplexed drug discovery campaigns.

Andras Boeszoermenyi
Denitsa L. Radeva
Haribabu Arthanari
Research05 May 2025
Nature Chemistry

Volume: 17, P: 835-846
Structural insights from lipid-bilayer nanodiscs link α-Synuclein membrane-binding modes to amyloid fibril formation

Thibault Viennet and colleagues gain structural insight into amyloid fibril formation from their innovative use of lipid bilayer nanodiscs. This study connects α-Synuclein membrane binding modes to its aggregation properties, furthering our understanding of the cause of neurodegerative diseases.

Thibault Viennet
Michael M. Wördehoff
Manuel Etzkorn
ResearchOpen Access03 May 2018
Communications Biology

Volume: 1, P: 1-12
The structural basis of PTEN regulation by multi-site phosphorylation

PTEN is a key cell signaling lipid phosphatase that is regulated by C-terminal phosphorylation. Biophysical methods were used to illuminate the structural basis for PTEN regulation, which involves a dynamic N-terminal helix that influences catalysis.

Daniel R. Dempsey
Thibault Viennet
Philip A. Cole
Research08 Oct 2021
Nature Structural & Molecular Biology

Volume: 28, P: 858-868
Protein semisynthesis reveals plasticity in HECT E3 ubiquitin ligase mechanisms

Lysine ubiquitination, catalysed by E3 ubiquitin ligases, is pivotal for regulating protein stability and cell signalling. Using protein semisynthesis, the roles of the C-terminal carboxylate and conformational interconversion in HECT-domain E3 catalysis are now characterized, revealing evolutionary plasticity in side chain versus backbone utilization.

Hanjie Jiang
Bryant D. Miller
Philip A. Cole
Research19 Jul 2024
Nature Chemistry

Volume: 16, P: 1894-1905
Structural basis of regulated m⁷G tRNA modification by METTL1–WDR4

Structures of the human METTL1–WDR4 complex are revealed, providing molecular insights into substrate recognition, modification and catalytic regulation by the N⁷-methylguanosine methyltransferase complex.

Jiazhi Li
Longfei Wang
Richard I. Gregory
Research04 Jan 2023
Nature

Volume: 613, P: 391-397
Targeting ROS production through inhibition of NADPH oxidases

NOXs are vital ROS-producing enzymes with roles in cell function and cancer. Here the authors combine computational and experimental methods to validate inhibitors for human NOX enzymes, opening avenues for redox biology-related cancer drug development.

Joana Reis
Christoph Gorgulla
Andrea Mattevi
Research26 Oct 2023
Nature Chemical Biology

Volume: 19, P: 1540-1550
Minimum information guidelines for experiments structurally characterizing intrinsically disordered protein regions

This Perspective introduces the Minimum Information About Disorder Experiments guidelines, which provide a community consensus on the minimum information required to appropriately describe metadata on experimentally and computationally derived structural state(s) of intrinsically disordered proteins or regions.

Bálint Mészáros
András Hatos
Norman E. Davey
Reviews03 Jul 2023
Nature Methods

Volume: 20, P: 1291-1303
Structural and dynamic insights revealing how lipase binding domain MD1 of Pseudomonas aeruginosa foldase affects lipase activation

Aldino Viegas
Peter Dollinger
Karl-Erich Jaeger
ResearchOpen Access27 Feb 2020
Scientific Reports

Volume: 10, P: 1-15

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Leveraging relaxation-optimized ¹H–¹³C_F correlations in 4-¹⁹F-phenylalanine as atomic beacons for probing structure and dynamics of large proteins

Structural insights from lipid-bilayer nanodiscs link α-Synuclein membrane-binding modes to amyloid fibril formation

The structural basis of PTEN regulation by multi-site phosphorylation

Protein semisynthesis reveals plasticity in HECT E3 ubiquitin ligase mechanisms

Structural basis of regulated m⁷G tRNA modification by METTL1–WDR4

Targeting ROS production through inhibition of NADPH oxidases

Minimum information guidelines for experiments structurally characterizing intrinsically disordered protein regions

Structural and dynamic insights revealing how lipase binding domain MD1 of Pseudomonas aeruginosa foldase affects lipase activation

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