It is challenging to localize synaptic proteins and dissect their functional domains in vivo. Here, the authors use the ALFA tag system to examine the trafficking and distribution of Drosophila Neurexin-1 and the role of the PDZ binding motif, and to accomplish its functional reconstitution in vivo.
- Rosario Vicidomini
- Saumitra Dey Choudhury
- Mihaela Serpe
