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Showing 1–3 of 3 results
Advanced filters: Author: Tia DiTommaso Clear advanced filters

  • Cas12i is a genome editing platform with compact size that fits in AAV vector with short 43-mer gRNA, absence of tracrRNA, ability to process pre-crRNA, and high specificity. Here the authors present an unbiased mutational scanning approach to engineer Cas12i, which shows low activity in mammalian cells, and identify single substitutions that significantly improve indel activity.

    • Colin McGaw
    • Anthony J. Garrity
    • Shaorong Chong
    ResearchOpen Access
    Nature Communications
    Volume: 13, P: 1-11

  • Large-scale efforts are put into the generation of knockout mutant mice for many individual genes. Here, the authors systematically screen skin from 538 mutant mice and identify 50 mutants with epidermal phenotypes, of which 9 are also associated with human skin defects.

    • Kifayathullah Liakath-Ali
    • Valerie E. Vancollie
    • Fiona M. Watt
    ResearchOpen Access
    Nature Communications
    Volume: 5, P: 1-13

  • In this study, the authors report the identification, biochemical characterization and cryo-electron microscopy structure of a phage-encoded miniature type V CRISPR-Cas nuclease in different functional states. This work provides mechanistic insight into CRISPR-Cas12 nucleases and a solid framework for future rational engineering.

    • Satoshi N. Omura
    • Lauren E. Alfonse
    • Osamu Nureki
    ResearchOpen Access
    Communications Biology
    Volume: 8, P: 1-15