Genetic selection uncovers a novel periplasmic chaperone called Spy, whose expression is strongly induced by conditions that cause protein unfolding. Spy can suppress protein aggregation and promote refolding in an ATP-independent manner in vitro. The crystal structure of Spy reveals a thin, cradle-shaped dimer that might shield aggregation-prone regions exposed in unfolded substrates.
- Shu Quan
- Philipp Koldewey
- James C A Bardwell
