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Showing 1–4 of 4 results
Advanced filters: Author: Tomoko Hirozane-Kishikawa Clear advanced filters
  • A combination of PCR stitching with next-generation sequencing results in Stitch-seq, a massively parallel method for interactome mapping. The approach is applied to human protein-protein interactions assayed in yeast two-hybrid screens.

    • Haiyuan Yu
    • Leah Tardivo
    • Marc Vidal
    Research
    Nature Methods
    Volume: 8, P: 478-480
  • A framework based on numerous empirical data, including protein-protein interaction reference sets, provides parameters for assessing the quality and coverage of protein-protein interaction datasets and estimation of the size of the human interactome. Braun et al., also in this issue, use the reference sets to help derive confidence scores for individual protein-protein interactions.

    • Kavitha Venkatesan
    • Jean-François Rual
    • Marc Vidal
    Research
    Nature Methods
    Volume: 6, P: 83-90
  • High-throughput yeast two-hybrid screening is used to generate the largest C. elegans interactome resource available thus far. Using an empirical quality control framework presented in Venkatesan et al., also online, the data set is evaluated for quality and is used to estimate the total size of the worm interactome.

    • Nicolas Simonis
    • Jean-François Rual
    • Marc Vidal
    Research
    Nature Methods
    Volume: 6, P: 47-54